Cell-line dependent effects of hypoxia prior to irradiation in squamous cell carcinoma lines.

ABSTRACT:

Purpose

To assess the impact of hypoxia exposure on cellular radiation sensitivity and survival of tumor cells with diverse intrinsic radiation sensitivity under normoxic conditions.

Materials and methods

Three squamous cell carcinoma (SCC) cell lines, with pronounced differences in radiation sensitivity, were exposed to hypoxia prior, during or post irradiation. Cells were seeded in parallel for colony formation assay (CFA) and stained for γH2AX foci or processed for western blot analysis.

Results

Hypoxia during irradiation led to increased cellular survival and reduced amount of residual γH2AX foci in all the cell lines with similar oxygen enhancement ratios (OER SKX: 2.31, FaDu: 2.44, UT-SCC5: 2.32), while post-irradiation hypoxia did not alter CFA nor residual γH2

SUBMITTER: Hauth F 

PROVIDER: S-EPMC5833923 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature