Single-cell RNA sequencing reveals gene expression signatures of breast cancer-associated endothelial cells.

ABSTRACT: Tumor endothelial cells (TEC) play an indispensible role in tumor growth and metastasis although much of the detailed mechanism still remains elusive. In this study we characterized and compared the global gene expression profiles of TECs and control ECs isolated from human breast cancerous tissues and reduction mammoplasty tissues respectively by single cell RNA sequencing (scRNA-seq). Based on the qualified scRNA-seq libraries that we made, we found that 1302 genes were differentially expressed between these two EC phenotypes. Both principal component analysis (PCA) and heat map-based hierarchical clustering separated the cancerous versus control ECs as two distinctive clusters, and MetaCore disease biomarker analysis indicated that these differentially expressed genes are highly correla

SUBMITTER: Sun Z 

PROVIDER: S-EPMC5834272 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature