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A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging.

ABSTRACT: Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with the examined structures and bivBC2-Nb staining results in a close-grained fluorophore labeling with minimal linkage errors. This allowed us to perform high-quality dSTORM imaging of various targets in mammalian and yeast cells. We expect that this versatile strategy will render many more demanding cellular targets amenable to dSTORM imaging.

SUBMITTER: Virant D

PROVIDER: S-EPMC5834503 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging.

Virant David D Traenkle Bjoern B Maier Julia J Kaiser Philipp D PD Bodenhöfer Mona M Schmees Christian C Vojnovic Ilijana I Pisak-Lukáts Borbála B Endesfelder Ulrike U Rothbauer Ulrich U

Nature communications 20180302 1

Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with the examined structures and bivBC2-Nb staining results in a close-grained fluorophore labeling with minimal linkage errors. This allowed us ...[more]

PMID: 29500346

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