A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging.

ABSTRACT: Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with the examined structures and bivBC2-Nb staining results in a close-grained fluorophore labeling with minimal linkage errors. This allowed us to perform high-quality dSTORM imaging of various targets in mammalian and yeast cells. We expect that this versatile strategy will render many more demanding cellular targets amenable to dSTORM imaging.

SUBMITTER: Virant D 

PROVIDER: S-EPMC5834503 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature