Culture-free Antibiotic-susceptibility Determination From Single-bacterium Raman Spectra.

ABSTRACT: Raman spectrometry appears to be an opportunity to perform rapid tests in microbiological diagnostics as it provides phenotype-related information from single bacterial cells thus holding the promise of direct analysis of clinical specimens without any time-consuming growth phase. Here, we demonstrate the feasibility of a rapid antibiotic-susceptibility determination based on the use of Raman spectra acquired on single bacterial cells. After a two-hour preculture step, one susceptible and two resistant E. coli strains were incubated, for only two hours, in the presence of different bactericidal antibiotics (gentamicin, ciprofloxacin, amoxicillin) in a range of concentrations that included the clinical breakpoints used as references in microbial diagnostic. Spectra were acquired and process

SUBMITTER: Novelli-Rousseau A 

PROVIDER: S-EPMC5834538 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature