Rewiring Yarrowia lipolytica toward triacetic acid lactone for materials generation.

ABSTRACT: Polyketides represent an extremely diverse class of secondary metabolites often explored for their bioactive traits. These molecules are also attractive building blocks for chemical catalysis and polymerization. However, the use of polyketides in larger scale chemistry applications is stymied by limited titers and yields from both microbial and chemical production. Here, we demonstrate that an oleaginous organism (specifically, Yarrowia lipolytica) can overcome such production limitations owing to a natural propensity for high flux through acetyl-CoA. By exploring three distinct metabolic engineering strategies for acetyl-CoA precursor formation, we demonstrate that a previously uncharacterized pyruvate bypass pathway supports increased production of the polyketide triacetic acid la

SUBMITTER: Markham KA 

PROVIDER: S-EPMC5834725 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature