Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis.

ABSTRACT:

Background

Equine piroplasmosis (EP) is an economically significant infection of horses and other equine species caused by the tick-borne protozoa Theileria equi and Babesia caballi. The long-term carrier state in infected animals makes importation of such subclinical cases a major risk factor for the introduction of EP into non-enzootic areas. Regulatory testing for EP relies on screening of equines by serological methods. The definitive diagnosis of EP infection in individual animals will benefit from the availability of sensitive direct detection methods, for example, when used as confirmatory assays for non-negative serological test results. The objectives of this study were to develop a real-time quantitative polymerase chain reaction (qPCR) assay for simultaneous detection of

SUBMITTER: Lobanov VA 

PROVIDER: S-EPMC5834856 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature