The Impact of the CD9 Tetraspanin on Lentivirus Infectivity and Exosome Secretion.

ABSTRACT: Efficient transduction tools are a hallmark for both research and therapy development. Here, we introduce new insights into the generation of lentiviral vectors with improved performance by utilizing producer cells with increased production rates of extracellular vesicles through CD9 overexpression. Most human cells secrete small vesicles from their surface (microvesicles) or intraluminal endosome-derived membranes (exosomes). In particular, enhanced levels of the tetraspanin CD9 result in significantly increased numbers of extracellular vesicles with exosome-like features that were secreted from four different human cell lines. Intriguingly, exosomes and their biogenesis route display similarities to lentivirus and we examined the impact of CD9 expression on release and infectivity of rec

SUBMITTER: Boker KO 

PROVIDER: S-EPMC5835022 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature