Transcriptional Innate Immune Response of the Developing Chicken Embryo to Newcastle Disease Virus Infection.

ABSTRACT: Traditional approaches to assess the immune response of chickens to infection are through animal trials, which are expensive, require enhanced biosecurity, compromise welfare, and are frequently influenced by confounding variables. Since the chicken embryo becomes immunocompetent prior to hatch, we here characterized the transcriptional response of selected innate immune genes to Newcastle disease virus (NDV) infection in chicken embryos at days 10, 14, and 18 of embryonic development. The results suggest that the innate immune response 72 h after challenge of 18-day chicken embryo is both consistent and robust. The expression of CCL5, Mx1, and TLR3 in lung tissues of NDV challenged chicken embryos from the outbred Kuroiler and Tanzanian local ecotype lines showed that their expression was

SUBMITTER: Schilling MA 

PROVIDER: S-EPMC5835104 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature