Imaging early embryonic calcium activity with GCaMP6s transgenic zebrafish.

ABSTRACT: Intracellular Ca²⁺ signaling regulates cellular activities during embryogenesis and in adult organisms. We generated stable Tg[βactin2:GCaMP6s]^stl351 and Tg[ubi:GCaMP6s]^stl352 transgenic lines that combine the ubiquitously-expressed Ca²⁺ indicator GCaMP6s with the transparent characteristics of zebrafish embryos to achieve superior in vivo Ca²⁺ imaging. Using the Tg[βactin2:GCaMP6s]^stl351 line featuring strong GCaMP6s expression from cleavage through gastrula stages, we detected higher frequency of Ca²⁺ transients in the superficial blastomeres during the blastula stages preceding the midblastula transition. Additionally, GCaMP6s also revealed that dorsal-biased Ca²⁺ signaling that follows the midblastu

SUBMITTER: Chen J 

PROVIDER: S-EPMC5835148 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature