Centriole Overduplication is the Predominant Mechanism Leading to Centrosome Amplification in Melanoma.

ABSTRACT: Centrosome amplification (CA) is common in cancer and can arise by centriole overduplication or by cell doubling events, including the failure of cell division and cell-cell fusion. To assess the relative contributions of these two mechanisms, the number of centrosomes with mature/mother centrioles was examined by immunofluorescence in a tissue microarray of human melanomas and benign nevi (n = 79 and 17, respectively). The centrosomal protein 170 (CEP170) was used to identify centrosomes with mature centrioles; this is expected to be present in most centrosomes with cell doubling, but on fewer centrosomes with overduplication. Using this method, it was determined that the majority of CA in melanoma can be attributed to centriole overduplication rather than cell doubling events. As Polo-li

SUBMITTER: Denu RA 

PROVIDER: S-EPMC5835182 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature