Transcriptome Analysis of Pseudomonas aeruginosa Cultured in Human Burn Wound Exudates.

ABSTRACT: Pseudomonas aeruginosa is a severe opportunistic pathogen and is one of the major causes of hard to treat burn wound infections. Herein we have used an RNA-seq transcriptomic approach to study the behavior of P. aeruginosa PAO1 growing directly on human burn wound exudate. A chemical analysis of compounds used by this bacterium, coupled with kinetics expression of central genes has allowed us to obtain a global view of P. aeruginosa physiological and metabolic changes occurring while growing on human burn wound exudate. In addition to the numerous virulence factors and their secretion systems, we have found that all iron acquisition mechanisms were overexpressed. Deletion and complementation with pyoverdine demonstrated that iron availability was a major limiting facto

SUBMITTER: Gonzalez MR 

PROVIDER: S-EPMC5835353 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature