Single-injection ex ovo transplantation method for broad spinal cord engraftment of human pluripotent stem cell-derived motor neurons.

ABSTRACT:

Background

Transplantation of human pluripotent stem cell (hPSC)-derived neurons into chick embryos is an established preliminary assay to evaluate engraftment potential. Yet, with recent advances in deriving diverse human neuronal subtypes, optimizing and standardizing such transplantation methodology for specific subtypes at their correlated anatomical sites is still required.

New method

We determined the optimal stage of hPSC-derived motor neuron (hMN) differentiation for ex ovo transplantation, and developed a single injection protocol that implants hMNs throughout the spinal cord enabling broad regional engraftment possibilities.

Results

A single injection into the neural tube lumen yielded a 100% chick embryo survival and successful transplantation rate with MN

SUBMITTER: Estevez-Silva MC 

PROVIDER: S-EPMC5835419 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature