Transcriptional Responses of Pseudomonas aeruginosa to Potable Water and Freshwater.

ABSTRACT: Many Pseudomonas aeruginosa infections are derived from residential, recreational, or surface water sources; thus, these environments represent an important preinfection niche. To better understand P. aeruginosa biology in these environments, we quantified transcriptional changes by microarray after exposure to diluted LB, diluted R2B, potable tap water, and freshwater from a eutrophic pond. Quantitative reverse transcription-PCR (qRT-PCR) confirmed the conservation of these responses in other water sources, and competition experiments were used to test the importance of three implicated metabolic pathways. The global transcriptional responses in potable water and freshwater showed strong induction of genes involved in metabolism of the head groups and acyl tails of phospholi

SUBMITTER: English EL 

PROVIDER: S-EPMC5835727 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature