Thermophilic Alkaline Fermentation Followed by Mesophilic Anaerobic Digestion for Efficient Hydrogen and Methane Production from Waste-Activated Sludge: Dynamics of Bacterial Pathogens as Revealed by the Combination of Metagenomic and Quantitative PCR Analyses.

ABSTRACT: Thermophilic alkaline fermentation followed by mesophilic anaerobic digestion (TM) for hydrogen and methane production from waste-activated sludge (WAS) was investigated. The TM process was also compared to a process with mesophilic alkaline fermentation followed by a mesophilic anaerobic digestion (MM) and one-stage mesophilic anaerobic digestion (M) process. The results showed that both hydrogen yield (74.5 ml H₂/g volatile solids [VS]) and methane yield (150.7 ml CH₄/g VS) in the TM process were higher than those (6.7 ml H₂/g VS and 127.8 ml CH₄/g VS, respectively) in the MM process. The lowest methane yield (101.2 ml CH₄/g VS) was obtained with the M process. Taxonomic results obtained from metagenomic analysis showed that differen

SUBMITTER: Wan J 

PROVIDER: S-EPMC5835733 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature