Epoxide hydrolase 1 (EPHX1) hydrolyzes epoxyeicosanoids and impairs cardiac recovery after ischemia.

ABSTRACT: Stimuli such as inflammation or hypoxia induce cytochrome P450 epoxygenase-mediated production of arachidonic acid-derived epoxyeicosatrienoic acids (EETs). EETs have cardioprotective, vasodilatory, angiogenic, anti-inflammatory, and analgesic effects, which are diminished by EET hydrolysis yielding biologically less active dihydroxyeicosatrienoic acids (DHETs). Previous in vitro assays have suggested that epoxide hydrolase 2 (EPHX2) is responsible for nearly all EET hydrolysis. EPHX1, which exhibits slow EET hydrolysis in vitro, is thought to contribute only marginally to EET hydrolysis. Using Ephx1^-/-, Ephx2^-/-, and Ephx1^-/-Ephx2^-/- mice, we show here that EPHX1 significantly contributes to EET hydrolys

SUBMITTER: Edin ML 

PROVIDER: S-EPMC5836130 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature