Systematic Identification of Lysine 2-hydroxyisobutyrylated Proteins in Proteus mirabilis.

ABSTRACT: Lysine 2-hydroxyisobutyrylation (K_hib) is a novel post-translational modification (PTM), which was thought to play a role in active gene transcription and cellular proliferation. Here we report a comprehensive identification of K_hib in Proteus mirabilis (P. mirabilis). By combining affinity enrichment with two-dimensional liquid chromatography and high-resolution mass spectrometry, 4735 2-hydroxyisobutyrylation sites were identified on 1051 proteins in P. mirabilis. These proteins bearing modifications were further characterized in abundance, distribution and functions. The interaction networks and domain architectures of these proteins with high confidence were revealed using bioinformatic tools. Our data demonstrate that many 2-hydroxyisobutyrylated pr

SUBMITTER: Dong H 

PROVIDER: S-EPMC5836373 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature