Expression pattern of Wif1 and β-catenin during development of anorectum in fetal rats with anorectal malformations.

ABSTRACT:

Purpose

This study was performed to investigate the expression pattern of Wnt inhibitory factor 1 (Wif1) and β-catenin during anorectal development in normal and anorectal malformation (ARM) embryos and the possible role of Wif1 and β-catenin in the pathogenesis of ARM.

Methods

ARM was induced with ethylenethiourea on the 10th gestational day in rat embryos. Cesarean deliveries were performed to harvest the embryos. The expression pattern of Wif1 and β-catenin protein and mRNA was evaluated in normal rat embryos (n = 288) and ARM rat embryos (n = 306) from GD13 to GD16 using immunohistochemical staining, Western blot, and real time RT-PCR.

Results

Immunohistochemical staining revealed that in normal embryos Wif1 was constantly expressed in the cloaca fro

SUBMITTER: Tang XB 

PROVIDER: S-EPMC5836566 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature