Integration of microRNA-mRNA profiles and pathway analysis of plant isoquinoline alkaloid berberine in SGC-7901 gastric cancers cells.

ABSTRACT:

Purpose

Berberine (BBR) is a traditional Chinese medicine normally used for gastroenteritis, and recent research found that it could fight against tumors. In this study, we focused on integrating miRNA sequencing and RNA sequencing of SGC-7901 gastric cancer cells treated by BBR to elucidate their underlying mechanisms.

Materials and methods

WST-1 assay and flow cytometry were used to check the effects of BBR on SGC-7901. miRNA sequencing and RNA sequencing were used to establish the miRNA and mRNA profiles of BBR-treated SGC-7901.

Results

The results showed that BBR could inhibit the proliferation of SGC-7901 cells and induce G1 arrest in cell cycle phase and apoptosis. A total of 1,960 upregulated genes and 4,837 downregulated genes were identified in the RNA sequen

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5836656 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature