Measurement of lipid transfer proteins in genetically engineered maize using liquid chromatography with tandem mass spectrometry (LC-MS/MS).

ABSTRACT: Endogenous allergenicity evaluation is a required part of the risk assessment for genetically engineered (GE) crops. Although maize is not considered a major allergenic food, a lipid transfer protein (Zea m 14) in maize grain has been identified as a potential IgE-mediated food allergen. Currently, the relationship between allergen exposure and risk of sensitization is not well understood. Hence, reliable quantitative methods are useful for determining the natural range and variability of allergen levels across multiple geographies and genetic backgrounds. A LC-MS/MS analytical method was developed and validated in our laboratory to quantify Zea m 14 in grain from 2 GE maize hybrids and 20 non-GE maize hybrids. The measured Zea m 14 levels in GE maize grain and conventional non-GE maize gr

SUBMITTER: Hill RC 

PROVIDER: S-EPMC5836814 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature