Decellularization of human donor aortic and pulmonary valved conduits using low concentration sodium dodecyl sulfate.

ABSTRACT: The clinical use of decellularized cardiac valve allografts is increasing. Long-term data will be required to determine whether they outperform conventional cryopreserved allografts. Valves decellularized using different processes may show varied long-term outcomes. It is therefore important to understand the effects of specific decellularization technologies on the characteristics of donor heart valves. Human cryopreserved aortic and pulmonary valved conduits were decellularized using hypotonic buffer, 0.1% (w/v) sodium dodecyl sulfate and nuclease digestion. The decellularized tissues were compared to cellular cryopreserved valve tissues using histology, immunohistochemistry, quantitation of total deoxyribose nucleic acid, collagen and glycosaminoglycan content, in vitro cytotoxicity ass

SUBMITTER: Vafaee T 

PROVIDER: S-EPMC5836965 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature