Interleukin-6 regulates expression of Fos and Jun genes to affect the development of mouse preimplantation embryos.

ABSTRACT:

Aim

We investigated whether recombinant mouse interleukin-6 (IL-6) affects the development of preimplantation embryos and induces the -signal transducers and activators of transcription (JAK-STAT) signaling pathway by binding IL-6 signal transducer (IL-6st) and regulating Fos and Jun gene expression, thereby accounting for the negative effect of superovulation on embryo development.

Methods

We compared rates of blastocyst formation from embryos cultured with different concentrations of IL-6 or/and anti-interleukin 6 receptor antibody (anti-IL-6RAb) in superovulated experimental and normal control groups. IL-6 expression in preimplantation embryos was determined by immunofluorescence identification. Fos, Jun and IL-6st messenger RNA expression was detected by PCR and microarr

SUBMITTER: Yu C 

PROVIDER: S-EPMC5836979 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature