Agreement in DNA methylation levels from the Illumina 450K array across batches, tissues, and time.

ABSTRACT: Epigenome-wide association studies (EWAS) have focused primarily on DNA methylation as a chemically stable and functional epigenetic modification. However, the stability and accuracy of the measurement of methylation in different tissues and extraction types is still being actively studied, and the longitudinal stability of DNA methylation in commonly studied peripheral tissues is of great interest. Here, we used data from two studies, three tissue types, and multiple time points to assess the stability of DNA methylation measured with the Illumina Infinium HumanMethylation450 BeadChip array. Redundancy analysis enabled visual assessment of agreement of replicate samples overall and showed good agreement after removing effects of tissue type, age, and sex. At the probe level, analysis of v

SUBMITTER: Forest M 

PROVIDER: S-EPMC5837078 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature