Noninvasive diagnosis of chronic kidney diseases using urinary proteome analysis.

ABSTRACT:

Background

In spite of its invasive nature and risks, kidney biopsy is currently required for precise diagnosis of many chronic kidney diseases (CKDs). Here, we explored the hypothesis that analysis of the urinary proteome can discriminate different types of CKD irrespective of the underlying mechanism of disease.

Methods

We used data from the proteome analyses of 1180 urine samples from patients with different types of CKD, generated by capillary electrophoresis coupled to mass spectrometry. A set of 706 samples served as the discovery cohort, and 474 samples were used for independent validation. For each CKD type, peptide biomarkers were defined using statistical analysis adjusted for multiple testing. Potential biomarkers of statistical significance were combined in suppo

SUBMITTER: Siwy J 

PROVIDER: S-EPMC5837301 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature