Characterization of primary human mammary epithelial cells isolated and propagated by conditional reprogrammed cell culture.

ABSTRACT:

Purpose

Conditional reprogramming methods allow for the inexhaustible in vitro proliferation of primary epithelial cells from human tissue specimens. This methodology has the potential to enhance the utility of primary cell culture as a model for mammary gland research. However, few studies have systematically characterized this method in generating in vitro normal human mammary epithelial cell models.

Results

We show that cells derived from fresh normal breast tissues can be propagated and exhibit heterogeneous morphologic features. The cultures are composed of CK18, desmoglein 3, and CK19-positive luminal cells and vimentin, p63, and CK14-positive myoepithelial cells, suggesting the maintenance of in vivo heterogeneity. In addition, the cultures contai

SUBMITTER: Jin L 

PROVIDER: S-EPMC5837767 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature