Improved protocols to accelerate the assembly of DNA barcode reference libraries for freshwater zooplankton.

ABSTRACT: Currently, freshwater zooplankton sampling and identification methodologies have remained virtually unchanged since they were first established in the beginning of the XX century. One major contributing factor to this slow progress is the limited success of modern genetic methodologies, such as DNA barcoding, in several of the main groups. This study demonstrates improved protocols which enable the rapid assessment of most animal taxa inhabiting any freshwater system by combining the use of light traps, careful fixation at low temperatures using ethanol, and zooplankton-specific primers. We DNA-barcoded 2,136 specimens from a diverse array of taxonomic assemblages (rotifers, mollusks, mites, crustaceans, insects, and fishes) from several Canadian and Mexican lakes with an average sequence

SUBMITTER: Elias-Gutierrez M 

PROVIDER: S-EPMC5838060 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature