Three dimensional electron microscopy reveals changing axonal and myelin morphology along normal and partially injured optic nerves.

ABSTRACT: Following injury to the central nervous system, axons and myelin distinct from the initial injury site undergo changes associated with compromised function. Quantifying such changes is important to understanding the pathophysiology of neurotrauma; however, most studies to date used 2 dimensional (D) electron microscopy to analyse single sections, thereby failing to capture changes along individual axons. We used serial block face scanning electron microscopy (SBF SEM) to undertake 3D reconstruction of axons and myelin, analysing optic nerves from normal uninjured female rats and following partial optic nerve transection. Measures of axon and myelin dimensions were generated by examining 2D images at 5 µm intervals along the 100 µm segments. In both normal and injured animals, changes in ax

SUBMITTER: Giacci MK 

PROVIDER: S-EPMC5838102 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature