Molecular cloning and expression analysis of annexin A2 gene in sika deer antler tip.

ABSTRACT:

Objective

Molecular cloning and bioinformatics analysis of annexin A2 (ANXA2) gene in sika deer antler tip were conducted. The role of ANXA2 gene in the growth and development of the antler were analyzed initially.

Methods

The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of the ANXA2 gene from antler tip of sika deer (Cervus Nippon hortulorum) and the bioinformatics methods were applied to analyze the amino acid sequence of Anxa2 protein. The mRNA expression levels of the ANXA2 gene in different growth stages were examined by real time reverse transcriptase polymerase chain reaction (real time RT-PCR).

Results

The nucleotide sequence analysis revealed an open reading frame of 1,020 bp e

SUBMITTER: Xia Y 

PROVIDER: S-EPMC5838317 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature