Multi-resistance strategy for viral diseases and in vitro short hairpin RNA verification method in pigs.

ABSTRACT: OBJECTIVE:Foot and mouth disease (FMD) and porcine reproductive and respiratory syndrome (PRRS) are major diseases that interrupt porcine production. Because they are viral diseases, vaccinations are of only limited effectiveness in preventing outbreaks. To establish an alternative multi-resistant strategy against FMD virus (FMDV) and PRRS virus (PRRSV), the present study introduced two genetic modification techniques to porcine cells. METHODS:First, cluster of differentiation 163 (CD163), the PRRSV viral receptor, was edited with the clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 technique. The CD163 gene sequences of edited cells and control cells differed. Second, short hairpin RNA (shRNAs) were integrated into the cells. The shRNAs, targeting the

SUBMITTER: Oh JN 

PROVIDER: S-EPMC5838320 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature