Hepatic transcriptional changes in critical genes for gluconeogenesis following castration of bulls.

ABSTRACT: OBJECTIVE:This study was performed to understand transcriptional changes in the genes involved in gluconeogenesis and glycolysis pathways following castration of bulls. METHODS:Twenty Korean bulls were weaned at average 3 months of age, and castrated at 6 months. Liver tissues were collected from bulls (n = 10) and steers (n = 10) of Korean cattle, and hepatic gene expression levels were measured using quantitative real-time polymerase chain reaction. We examined hepatic transcription levels of genes encoding enzymes for irreversible reactions in both gluconeogenesis and glycolysis as well as genes encoding enzymes for the utilization of several glucogenic substrates. Correlations between hepatic gene expression and carcass characteristics were performed to understand their associations. R

SUBMITTER: Fassah DM 

PROVIDER: S-EPMC5838326 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature