Interleukin-17 regulates matrix metalloproteinase activity in human pulmonary tuberculosis.

ABSTRACT: Tuberculosis (TB) is characterized by extensive pulmonary matrix breakdown. Interleukin-17 (IL-17) is key in host defence in TB but its role in TB-driven tissue damage is unknown. We investigated the hypothesis that respiratory stromal cell matrix metalloproteinase (MMP) production in TB is regulated by T-helper 17 (T_H -17) cytokines. Biopsies of patients with pulmonary TB were analysed by immunohistochemistry (IHC), and patient bronchoalveolar lavage fluid (BALF) MMP and cytokine concentrations were measured by Luminex assays. Primary human airway epithelial cells were stimulated with conditioned medium from human monocytes infected with Mycobacterium tuberculosis (Mtb) and T_H -17 cytokines. MMP secretion, activity, and gene expression were determined by ELISA, Lumin

SUBMITTER: Singh S 

PROVIDER: S-EPMC5838784 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature