Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization.

ABSTRACT:

Background

Proteomic analyses of clinical specimens often rely on human tissues preserved through formalin-fixation and paraffin embedding (FFPE). Minimal sample consumption is the key to preserve the integrity of pathological archives but also to deal with minimal invasive core biopsies. This has been achieved by using the acid-labile surfactant RapiGest in combination with a direct trypsinization (DTR) strategy. A critical comparison of the DTR protocol with the most commonly used filter aided sample preparation (FASP) protocol is lacking. Furthermore, it is unknown how common histological stainings influence the outcome of the DTR protocol.

Methods

Four single consecutive murine kidney tissue specimens were prepared with the DTR approach or with the FASP protocol using bo

SUBMITTER: Foll MC 

PROVIDER: S-EPMC5838928 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature