Summarizing performance for genome scale measurement of miRNA: reference samples and metrics.

ABSTRACT:

Background

The potential utility of microRNA as biomarkers for early detection of cancer and other diseases is being investigated with genome-scale profiling of differentially expressed microRNA. Processes for measurement assurance are critical components of genome-scale measurements. Here, we evaluated the utility of a set of total RNA samples, designed with between-sample differences in the relative abundance of miRNAs, as process controls.

Results

Three pure total human RNA samples (brain, liver, and placenta) and two different mixtures of these components were evaluated as measurement assurance control samples on multiple measurement systems at multiple sites and over multiple rounds. In silico modeling of mixtures provided benchmark values for comparison with physical m

SUBMITTER: Pine PS 

PROVIDER: S-EPMC5838960 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature