Successful use of whole genome amplified DNA from multiple source types for high-density Illumina SNP microarrays.

ABSTRACT:

Background

The recommended genomic DNA input requirements for whole genome single nucleotide polymorphism microarrays can limit the scope of molecular epidemiological studies. We performed a large-scale evaluation of whole genome amplified DNA as input into high-density, whole-genome Illumina® Infinium® SNP microarray.

Results

Overall, 6622 DNA samples from 5970 individuals were obtained from three distinct biospecimen sources and genotyped using gDNA and/or wgaDNA inputs. When genotypes from the same individual were compared with standard, native gDNA input amount, we observed 99.94% mean concordance with wgaDNA input.

Conclusions

Our results demonstrate that carefully conducted studies with wgaDNA inputs can yield high-quality genotyping results. These findings shou

SUBMITTER: Dagnall CL 

PROVIDER: S-EPMC5838969 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature