MiR-190 suppresses breast cancer metastasis by regulation of TGF-β-induced epithelial-mesenchymal transition.

ABSTRACT: BACKGROUND:Breast cancer is the most common cancer among women worldwide and metastasis is the leading cause of death among patients with breast cancer. The transforming growth factor-β (TGF-β) pathway plays critical roles during breast cancer epithelial-mesenchymal transition (EMT) and metastasis. SMAD2, a positive regulator of TGF-β signaling, promotes breast cancer metastasis through induction of EMT. METHODS:The expression of miR-190 and SMAD2 in breast cancer tissues, adjacent normal breast tissues and cell lines were determined by RT-qPCR. The protein expression levels and localization were analyzed by western blotting and immunofluorescence. ChIP and dual-luciferase report assays were used to validate the regulation of ZEB1-miR-190-SMAD2 axis. The effect of miR-190 on breast cancer

SUBMITTER: Yu Y 

PROVIDER: S-EPMC5838994 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature