Cell Membrane Tracking in Living Brain Tissue Using Differential Interference Contrast Microscopy.

ABSTRACT: Differential interference contrast (DIC) microscopy is widely used for observing unstained biological samples that are otherwise optically transparent. Combining this optical technique with machine vision could enable the automation of many life science experiments; however, identifying relevant features under DIC is challenging. In particular, precise tracking of cell boundaries in a thick ( ) slice of tissue has not previously been accomplished. We present a novel deconvolution algorithm that achieves the state-of-the-art performance at identifying and tracking these membrane locations. Our proposed algorithm is formulated as a regularized least squares optimization that incorporates a filtering mechanism to handle organic tissue interference and a robust edge-sparsity regularizer that i

SUBMITTER: Lee J 

PROVIDER: S-EPMC5839128 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature