A Reduced Transcriptome Approach to Assess Environmental Toxicants Using Zebrafish Embryo Test.

ABSTRACT: Omics approaches can monitor responses and alterations of biological pathways at genome-scale, which are useful to predict potential adverse effects by environmental toxicants. However, high throughput application of transcriptomics in chemical assessment is limited due to the high cost and lack of "standardized" toxicogenomic methods. Here, a reduced zebrafish transcriptome (RZT) approach was developed to represent the whole transcriptome and to profile bioactivity of chemical and environmental mixtures in zebrafish embryo. RZT gene set of 1637 zebrafish Entrez genes was designed to cover a wide range of biological processes, and to faithfully capture gene-level and pathway-level changes by toxicants compared with the whole transcriptome. Concentration-response modeling was used to calcul

SUBMITTER: Wang P 

PROVIDER: S-EPMC5839301 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature