TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis.

ABSTRACT: During cytokinesis, a signal from the central spindle that forms between the separating anaphase chromosomes promotes the accumulation of contractile ring components at the cell equator, while a signal from the centrosomal microtubule asters inhibits accumulation of contractile ring components at the cell poles. However, the molecular identity of the inhibitory signal has remained unknown. To identify molecular components of the aster-based inhibitory signal, we developed a means to monitor the removal of contractile ring proteins from the polar cortex after anaphase onset. Using this assay, we show that polar clearing is an active process that requires activation of Aurora A kinase by TPXL-1. TPXL-1 concentrates on astral microtubules coincident with polar clearing in anaphase, and its ab

SUBMITTER: Mangal S 

PROVIDER: S-EPMC5839786 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature