Sialic acid-binding immunoglobulin-like lectin 8 (Siglec-8) is an activating receptor mediating β₂-integrin-dependent function in human eosinophils.

ABSTRACT:

Background

Siglec-8 is a CD33 subfamily cell-surface receptor selectively expressed on human eosinophils. After cytokine priming, Siglec-8 mAb or glycan ligand binding causes eosinophil apoptosis associated with reactive oxygen species (ROS) production. Most CD33-related Siglecs function as inhibitory receptors, but the ability of Siglec-8 to stimulate eosinophil ROS production and apoptosis suggests that Siglec-8 might instead function as an activating receptor.

Objective

We sought to determine the role of IL-5 priming and identify the signaling molecules involved in Siglec-8 function for human eosinophils.

Methods

We used an mAb and/or a multimeric synthetic sulfated sialoglycan ligand recognizing Siglec-8 in combination with integrin blocking antibodies, pharmacolo

SUBMITTER: Carroll DJ 

PROVIDER: S-EPMC5839929 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature