Rb is required for retinal angiogenesis and lamination.

ABSTRACT: Retinoblastoma tumor suppressor (Rb) promotes cell cycle exit, survival, differentiation, and tumor suppression in the retina. Here, we show it is also essential for vascularization and lamination. Despite minimal effects on Hif1a target expression, intraretinal vascular plexi did not form in the Rb ^-/- murine retina. Deleting adenovirus E2 promoter binding factor 3 (E2f3), which rescues starburst amacrine cell differentiation, or E2f2, had no effect, but deleting E2f1, which promotes neuronal cell cycle exit and survival, restored retinal vasculature. We specifically linked cell loss to the defect because removing Bax rescued rod and bipolar neurons and the vasculature, but not cell cycle exit. Despite rescuing Rb ^-/- neurons, Bax deletion exacerbated a delay in oute

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC5840357 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature