Application of maltose as energy source in protein-free CHO-K1 culture to improve the production of recombinant monoclonal antibody.

ABSTRACT: Oligosaccharides are generally considered to be un-utilized for growth of mammalian cells because their permeability across the cell membrane is low. However, in our previous study, we discovered that CHO and HEK293 cells consume maltose in culture media without serum and glucose. This is interesting because the transporter for maltose in mammalian cells has not been discovered to-date, and the only animal disaccharide transporter that is recently discovered is a sucrose transporter. The application of oligosaccharides in mammalian cell-based biopharmaceutical manufacturing can be beneficial, because it can theoretically increase carbohydrate content of the culture medium and decrease lactate production. Here, we first determined that specific maltose consumption rate in CHO cells was simi

SUBMITTER: Leong DSZ 

PROVIDER: S-EPMC5840386 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature