Long noncoding RNA RP4 functions as a competing endogenous RNA through miR-7-5p sponge activity in colorectal cancer.

ABSTRACT:

Aim

To investigate the role of long noncoding RNA (lncRNA) RP4 in colorectal cancer.

Methods

Lentivirus-mediated lncRNA RP4 overexpression and knockdown were performed in the colorectal cancer cell line SW480. Cell proliferation, tumor growth, and early apoptosis were evaluated by a cell counting kit-8 assay, an in vivo xenograft tumor model, and annexin V/propidium iodide staining, respectively. Analysis of the lncRNA RP4 mechanism involved assessment of the association of its expression with miR-7-5p and the SH3GLB1 gene. Western blot analysis was also performed to assess the effect of lncRNA RP4 on the autophagy-mediated cell death pathway and phosphatidylinositol-3-kinase (PI3K)/Akt signaling.

Results

Cell proliferation, tumor growth, and early apopt

SUBMITTER: Liu ML 

PROVIDER: S-EPMC5840465 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature