A novel ex vivo method for measuring whole brain metabolism in model systems.

ABSTRACT: BACKGROUND:Many neuronal and glial diseases have been associated with changes in metabolism. Therefore, metabolic reprogramming has become an important area of research to better understand disease at the cellular level, as well as to identify targets for treatment. Model systems are ideal for interrogating metabolic questions in a tissue dependent context. However, while new tools have been developed to study metabolism in cultured cells there has been less progress towards studies in vivo and ex vivo. NEW METHOD:We have developed a method using newly designed tissue restraints to adapt the Agilent XFe96 metabolic analyzer for whole brain analysis. These restraints create a chamber for Drosophila brains and other small model system tissues to reside undisrupted, while still remaining in t

SUBMITTER: Neville KE 

PROVIDER: S-EPMC5840572 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature