Evolution of H9N2 avian influenza virus in embryonated chicken eggs with or without homologous vaccine antibodies.

ABSTRACT:

Background

Vaccines constitute a unique selective pressure, different from natural selection, drives the evolution of influenza virus. In this study, A/Chicken/Shanghai/F/1998 (H9N2) was continually passaged in specific pathogen-free embryonated chicken eggs with or without selective pressures from antibodies induced by homologous maternal antibodies. Genetic mutations, antigenic drift, replication, and pathogenicity of the passaged virus were evaluated.

Results

Antigenic drift of the passaged viruses occurred in the 47th generation (vF47) under selective pressure on antibodies and in the 52nd generation (nF52) without selective pressure from antibodies. Seven mutations were observed in the vF47 virus, with three in PB2 and four in HA, whereas 12 mutations occurred in the nF

SUBMITTER: Jin H 

PROVIDER: S-EPMC5840701 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature