Molecular and biochemical responses in the midgut of the silkworm, Bombyx mori, infected with Nosema bombycis.

ABSTRACT:

Background

Microsporidia are a group of eukaryotic intracellular parasites that infect almost all vertebrates and invertebrates. However, there is little information available of how microsporidia obtain nutrients and energy from host cells. The purpose of this study was to investigate the energy and material requirements of Nosema bombycis for the invasion procedure through analyzing the global variation of the gene expression, protein abundance, fatty acids level and ATP flux induced by the microsporidia N. bombycis infection in the midgut of the silkworm Bombyx mori.

Methods

A suppression subtractive hybridization (SSH) and quantitative real-time PCR (qPCR) analysis were performed to identify the genes upregulated in the midgut of B. mori 48 h following N. bombycis infect

SUBMITTER: Li Z 

PROVIDER: S-EPMC5840838 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature