Inter-niche and inter-individual variation in gut microbial community assessment using stool, rectal swab, and mucosal samples.

ABSTRACT: The purpose of this study is to evaluate similarities and differences in gut bacterial measurements and stability in the microbial communities of three different types of samples that could be used to assess different niches of the gut microbiome: rectal swab, stool, and normal rectal mucosa samples. In swab-stool comparisons, there were substantial taxa differences with some taxa varying largely by sample type (e.g. Thermaceae), inter-individual subject variation (e.g. Desulfovibrionaceae), or by both sample type and participant (e.g. Enterobacteriaceae). Comparing all three sample types with whole-genome metagenome shotgun sequencing, swab samples were much closer to stool samples than mucosa samples although all KEGG functional Level 1 and Level 2 pathways were significantly different a

SUBMITTER: Jones RB 

PROVIDER: S-EPMC5841359 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature