Quantitative super-resolution single molecule microscopy dataset of YFP-tagged growth factor receptors.

ABSTRACT:

Background

Super-resolution single molecule localization microscopy (SMLM) is a method for achieving resolution beyond the classical limit in optical microscopes (approx. 200 nm laterally). Yellow fluorescent protein (YFP) has been used for super-resolution single molecule localization microscopy, but less frequently than other fluorescent probes. Working with YFP in SMLM is a challenge because a lower number of photons are emitted per molecule compared with organic dyes, which are more commonly used. Publically available experimental data can facilitate development of new data analysis algorithms.

Findings

Four complete, freely available single molecule super-resolution microscopy datasets on YFP-tagged growth factor receptors expressed in a human cell line are presented, i

SUBMITTER: Lukes T 

PROVIDER: S-EPMC5841371 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature