Multiscale analysis of a regenerative therapy for treatment of volumetric muscle loss injury.

ABSTRACT: Skeletal muscle possesses a remarkable capacity to regenerate when injured, but when confronted with major traumatic injury resulting in volumetric muscle loss (VML), the regenerative process consistently fails. The loss of muscle tissue and function from VML injury has prompted development of a suite of therapeutic approaches but these strategies have proceeded without a comprehensive understanding of the molecular landscape that drives the injury response. Herein, we administered a VML injury in an established rodent model and monitored the evolution of the healing phenomenology over multiple time points using muscle function testing, histology, and expression profiling by RNA sequencing. The injury response was then compared to a regenerative medicine treatment using orthotopic transpla

SUBMITTER: Aguilar CA 

PROVIDER: S-EPMC5841404 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature