Evolutionary Characterization of the Retinitis Pigmentosa GTPase Regulator Gene.

ABSTRACT:

Purpose

The evolutionary conservation of the retinitis pigmentosa GTPase regulator (RPGR) gene was examined across vertebrate and invertebrate lineages to elucidate its function.

Methods

Orthologous RPGR sequences from vertebrates and invertebrates were selected. Multiple sequence alignments, phylogenetic analyses, synteny, and gene structure comparisons were carried out. Expression of the alternatively spliced constitutive (RPGR(const) or RPGR(ex1-19)) and RPGR(ORF15) isoforms was examined in developing and adult zebrafish.

Results

Phylogenetic analyses and syntenic relationships were consistent with the selected sequences being true orthologues, although whole genome duplications in teleost fish resulted in a more complex picture. The splice form RPGR(const) was pre

SUBMITTER: Raghupathy RK 

PROVIDER: S-EPMC5841567 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature