Orosomucoid-like 3 (ORMDL3) upregulates airway smooth muscle proliferation, contraction, and Ca²⁺ oscillations in asthma.

ABSTRACT:

Background

Airway hyperresponsiveness is a major feature of asthma attributed predominantly to an extrinsic immune/inflammatory response increasing airway smooth muscle (ASM) contractility.

Objective

We investigated whether increased ASM expression of orosomucoid-like 3 (ORMDL3), a gene on chromosome 17q21 highly linked to asthma, induced increased ASM proliferation and contractility in vitro and influenced airway contractility and calcium flux in ASM in precision-cut lung slices (PCLSs) from wild-type and hORMDL3^Zp3-Cre mice (which express increased levels of human ORMDL3 [hORMDL3]).

Methods

Levels of ASM proliferation and contraction were assessed in ASM cells transfected with ORMDL3 in vitro. In addition, airway contractility and calcium oscillations wer

SUBMITTER: Chen J 

PROVIDER: S-EPMC5842097 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature