Conservation of Dynamics Associated with Biological Function in an Enzyme Superfamily.

ABSTRACT: Enzyme superfamily members that share common chemical and/or biological functions also share common features. While the role of structure is well characterized, the link between enzyme function and dynamics is not well understood. We present a systematic characterization of intrinsic dynamics of over 20 members of the pancreatic-type RNase superfamily, which share a common structural fold. This study is motivated by the fact that the range of chemical activity as well as molecular motions of RNase homologs spans over 10⁵ folds. Dynamics was characterized using a combination of nuclear magnetic resonance experiments and computer simulations. Phylogenetic clustering led to the grouping of sequences into functionally distinct subfamilies. Detailed characterization of the diverse RN

SUBMITTER: Narayanan C 

PROVIDER: S-EPMC5842143 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature